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FFPE Samples Human Tissue Specimen Preparation

Many researchers currently prefer to use FFPE tissue samples for their IHC, histological, or in situ genome analysis. FFPE stands for "Formalin-Fixed Paraffin-Embedded" and describes the two main features of this network storage method. Formalin is a formaldehyde solution and has been used since the discovery of the conservative effect of formaldehyde in the late 19th century by the German physician Ferdinand Bloom. 

After 'fixation of FFPE block storage with formaldehyde', the paraffin is introduced into the tissue, and the tissue is also surrounded by a paraffin shell to support it and prevent oxidation. Professionally mounted and embedded FFPE samples and the biomolecules they contain are relatively stable at room temperature. 

What Is FFPE Tissue And What Are Its Uses - BioChain Institute Inc.

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Structurally, the fixed and embedded tissues are elastic and can be used almost indefinitely for microscopic anatomical studies. With the passage of time, the antigenicity of some proteins will decrease, limiting IHC studies to samples no more than a few decades old. 

Archives that collect large numbers of FFPE samples are a particularly rich source of data when multi-case comparisons are desired to draw statistically stable conclusions about the nature of a particular indication. If FFPE specimens are to be used in high-use biomedical research, it is important that each stage of the preparation process is carried out by a trained and certified professional.

Before the tissue is processed, the necessary step is the removal of the tissue. This is indeed the most difficult element of control as it relates to patient care. The specifics of the medical procedure and standard care conventions are important because they can affect variables such as the time interval between anesthesia and vascular ligation, removal of tissue, and the elapsed time for fixation, all of which can affect specimen quality.