The adrenal cortex produces and secretes cortisol, the primary glucocorticoid. Cortisol is sometimes called the "stress hormone", as it plays a role in stress response and affects blood sugar and blood pressure. Cortisol is an immunological anti-inflammatory that plays a key role in hypersensitivity, immunosuppression and disease resistance.
Cortisol is a metabolic stimulant. It promotes liver glycogen deposition and gluconeogenesis. Cortisol Enzyme immunoassay kit is used to quantify cortisol levels in saliva, urine, serum and plasma. You can buy cortisol eia kits via https://www.bosterbio.com/cortisol-eia-kit-elisa-kit-ek7119-boster.html.
The ACTH-dependent circadian rhythm dictates that cortisol production peaks in the morning, and then decreases throughout the day. The majority of serum cortisol (less than 4%) is bound to proteins such as corticosteroid binding globulin or serum albumin. Most receptors only have free cortisol. It is through these receptors that physiological functions are controlled.
This kit measures total cortisol levels in serum, plasma, extracted samples, and free cortisol found in saliva and urine. To create a standard curve, a cortisol reference is included. To capture mouse antibodies, standard or diluted samples can be pipetted into a microtiter plate coated in an antibody.
The wells are then coated with a cortisol-peroxidase combinationate. Each well is then initiated by adding a monoclonal antibody against cortisol. After an hour of incubation, the plate is washed. Substrate is then added. The substrate reacts to the bound cortisol peroxidase conjugate. After a brief incubation, the color reaction is measured at 450 nm.